Care must be taken when using a blood cell analyzer

Care must be taken when using a blood cell analyzer

　　 In order to ensure that the results obtained by using the hematology analyzer can reflect the true situation of the patient as much as possible, the monitor manufacturer must pay attention to the following aspects when using it:

　　 1. Blood sample: Because venous blood is less affected by external factors, the composition is relatively stable, and the test results are highly accurate and reproducible. Therefore, except for infants, monitor manufacturers recommend that blood should be used for blood sampling. If collecting un-apparent blood, be careful not to squeeze it locally to avoid mixing a large amount of tissue fluid in the blood, and it is easy to activate the coagulation system to produce local coagulation, resulting in errors in the test results; the first drop of blood should be discarded due to unstable cell composition. Use the second drop of blood for testing.

2. Anticoagulation: The use of citrate anticoagulant for too long is easy to crystallize, and the morphology of cells is prone to change, which affects the accuracy of the counting results; oxalate is easy to cause platelet aggregation and can change the morphology of white blood cells. Counting results and classification; excessive heparin anticoagulation is likely to cause leukocyte aggregation and thrombocytopenia; EDTA-2Na has lower solubility than EDTA-2K, and the possibility of platelet aggregation is greater. Therefore, the document published by the International Committee of Hematology Standards (ICSH) in 1993 recommended the use of EDTA-2K as an anticoagulant for blood cell analyzers, with a dosage of 1.5 to 2.2 mg/ml blood.

　　 3. After blood collection, seal with a stopper and store at room temperature for no more than 6 hours.

　　 4. Dilution: The diluter and suction tube should be calibrated. After sucking blood, the blood outside the suction tube should be completely wiped off. The blood should be measured as soon as possible after dilution, otherwise it is easy to cause "diluted hemolysis".

　　 5. Mixing: Mixing before testing is very important, if there is no rotating mixer, it should be mixed upside down at least 8 times.

　　 6. Reagents: Blood cell analyzers have very strict requirements on reagents, which require strict osmotic pressure standards, stable conductivity, high standard purity, and no corrosive effect on instrument pipes and valve circuits. Therefore, hemolytic agents, diluents and cleaning agents are best selected from the original supporting products.

　　 7. White blood cell classification: First of all, it must be clear that so far, no matter how many advanced blood cell analyzers in the world, the white blood cell classification is only a screening method, and it cannot completely replace artificial microscopic classification. Monitor manufacturers must resolutely correct the erroneous thinking that some units use a hematology analyzer and lose the microscopic examination.

　　 8. Quality control: The blood cell analyzer must establish a strict quality control system to ensure the reliability of the results.